General Cell Based Assays
Aat Bioquest Cal-590™ AM, 10x50µg
Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3 filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor 488 labeled antibodies.
Bioassay Systems EnzyChrom™ Aconitase Assay Kit. For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.
Revvity Health Sciences Inc LANCE Ultra cAMP Detection Kit, 50,000 Assay Points
LANCE Ultra cAMP Detection Kit, 50,000 Assay Points
ELABSCIENCE Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method) (E-BC-K109-M) 48 Tests
Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce hydrogen peroxide. In the presence of Trinder's reagent, hydrogen peroxide is catalyzed by peroxidase (POD) to produce a red quinoneimine dye, whose color intensity is directly proportional to the TC concentration and can be measured at 510 nm.
Cayman Chemical NFAT Inhibitor, Purity: ≥95%, CAS: 249537-73-3, 1 mg
A peptide inhibitor of NFAT; binds to the NFAT recognition site on calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity at 100 µM; inhibits the dephosphorylation of NFAT in HEK293T lysates expressing NFAT1, -2, or -4; inhibits NFAT activation in RAW 264.7 macrophages, EA.hy926 endothelial cells, and isolated mouse thoracic aorta VSMCs (IC50s = 29.1, 30.2, and 45.7 µM, respectively, in reporter assays)
Cayman Chemical Dihydroethidium, Purity: ≥98%, CAS: 104821-25-2, 10 mg
A fluorescent dye to monitor superoxide production; ex/em = 490/590 nm; has been used to detect ROS and intracellular superoxide in vitro
MOLECULAR DEPOT LLC Conjugated Bilirubin - 1 mL
Conjugated Bilirubin for Interference Testing a highly concentrated solution of conjugated bilirubin (20 mg/mL) provided with the corresponding diluent This solution can be spiked into specimens to test conjugated bilirubin interference Instructions with sample calculations are also provided For in vitro research use only
Eurofins Discoverx PathHunter Bioassay Detection Kit, 75 (1,000 dp, 96-well). (93-0933)
The PathHunter Bioassay Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells or cell lines. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0933.
Abcam Swinholide A, F-actin inhibitor, 10UG
MW 1389.9 Da, Purity >97%. Cell permeable, selective F-actin inhibitor. Sequesters and stabilizes actin dimers. Induces actin depolymerization and apoptosis. .
The product is subject to the following: Abcam Restricted Use Statement