General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay, 2-plate Kit. (96-0009E3CP16M)
The InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2L1 target in an untagged form and Bax binding partner fused to ProLabel. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GALR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0058E2CP2M)
The cAMP Hunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GALR1, a naturally Gi/Go coupled GPCR. Upon activation of GALR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human MET soluble ASY
The R-PLEX Human Met Soluble Assay from Meso Scale Discovery is a multiplex immunoassay that measures 10 metabolic biomarkers in human serum plasma and cell culture supernatant It uses electrochemiluminescence detection for high sensitivity and broad dynamic range with low sample volume The panel includes insulin leptin PAI-1 total resistin MCP-1 adiponectin TNF-alpha IL-6 CRP and visfatin The assay provides efficient analysis of metabolic and inflammatory status with a protocol time of just over 3 hours It includes a 96-well plate with capture antibodies detection antibodies and calibrators For research use only
Cayman Chemical TGR5GP-BAR1 Reporter asy 1ea
An IRE1a inhibitor (IC50s 0.2 and 0.02 UM for RNase and kinase activity respectively) selective for IRE1a over Ron and FGFR1 (IC50s 4.4 and 17 UM) as well as a panel of 282 kinases at 10 UM inhibits ER stress-induced increases in the splicing of mRNA encoding XBP1 in eight multiple myeloma cell lines at 8 UM reduces viral replication in tick-borne encephalitis virus-infected SH-SY5Y and SNB-19 cells at 0.5 UM and tick-borne encephalitis virus-infected Caco-2 cells at 1 UM
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.
Eurofins Discoverx PathHunter eXpress mADCYAP1R1 (PAC1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0594E2MCP0M)
The PathHunter mADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of mADCYAP1R1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mADCYAP1R1 and EA-tagged β-Arrestin-2. Activation of mADCYAP1R1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR2 Activated GPCR Internalization Assay, 10pl Kit. (93-0677E3CP0L)
The PathHunter TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TACR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR2 Activated GPCR Internalization Assay, 2pl Kit. (93-0676E2CP0M)
The PathHunter SSTR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of SSTR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR173 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0380E2ACP1L)
The PathHunter GPR173 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR173 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR173 and EA-tagged β-Arrestin-2. Activation of GPR173 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0235E3CP0M)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress C5AR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0079E2CP2L)
The cAMP Hunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type C5AR1, a naturally Gi/Go coupled GPCR. Upon activation of C5AR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0076E2CP2M)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.